Anaerocult® A mini

1.10641.0007


For generating an anaerobic environment in one to four Petri dishes to permit cultivation of obligate and facultative anaerobes.

Anaerocult® A mini contains components which chemically bind oxygen quickly and completely, creating an oxygen-free (anaerobic) environment and a CO2 atmosphere.

Contents
 25 Anaerocult® A mini sachets
25 special incubation bags

Composition of Sachet Reactants
Components % by weight
Kieselguhr < 60%
Iron Powder < 30%
Citric Acid < 20%
Sodium Carbonate < 10%

The chemical mixture inside the sachet contains free crystalline silica. In case of damage to the sachet do not inhale dust. Repeated inhalations can cause severe harm to health. Contact with the eyes may cause irritations.

Materials Provided
 25 Anaerocult® A mini sachets
25 special incubation bags
Materials Required but Not Provided
 Anaerotest® strips (Cat. No. 1.13675)
10ml pipet
Deionized water
Adhesive tape
Anaeroclip® (Cat. No. 14255) or heat sealer

Quality Control
 Anaerobiosis is indicated by the color change in the Anaerotest® strip from blue to white within four hours.* Stock anaerobic organisms may also be used.

* Anaerobic conditions are achieved in about one hour. This is well before the 3-4 hours it takes for the indicator strip to decolorize.

Procedure for In Vitro Diagnostic Use

Figure 1
1. Moisten the reaction zone of an Anaerotest® strip (Cat. No. 1.13675) with water.
Figure 2
2. Stick the Anaerotest® strip onto the lid of one of the inoculated Petri dishes (the reaction zone must point downwards and hang freely in the open space).

Figure 3
3. Place the Anaerocult® A mini sachet into a special incubation bag.
Figure 4
4. Moisten the Anaerocult® A mini sachet with 8.0ml of water.

Figure 5
5. Place the Petri dish(es) immediately into the special incubation bag and close with Anaeroclip® (Cat. No. 14255) or seal with an ordinary plastic heat sealer. (It is advisable to seal with a double seal.) The bag must be sealed closed approximately 2 cm from the opening.

Stability
 See expiration date.

Storage
 Seal tightly and protect from moisture (seal the plastic bag well after removing each Anaerocult® A mini sachet). Recommended storage temperature is +15°C to +25°C.