Application
For the detection of an anaerobic
atmosphere.
Principle
The blue oxidized form of the dye
methylene blue is converted in an oxygen-free (anaerobic) medium inot the (colorless)
leucomethylene blue. In the presence of oxygen the reduced leucobase passes again into the
oxidized form (blue).
Composition
Methylene blue - Reducing agent - Stabilizer
Directions
The indicator is used together
with Cat. No. 13677, Anaerocult® A in the anaerobic jar, with Cat. No. 13676.
Anaerocult® A mini for up to 4 Petri dishes. Anaerotest® can also
be used with other anaerobiosis systems.
Procedure
Moisten the reaction zone with one drop of distilled water and put Anaerotest® into
the anaerobic jar.
When sing Cat. No. 13677, Anaerocult® A and Cat No. 1.10641.0007, Anaerocult® A
mini, draw exact instructions from the respective package inserts.
Evaluation
In an anaerobic atmosphere
the reaction zone is decolorized in about four hours* (change of color from blue to white).
*Anaerobic conditions while using Anaerocult® A (Cat. No. 13677) are achieved
in approximately one hour. This is well before the 3-4 hours it takes for the indicator strip
to decolorize.
Stability
See expiration date.
Storage
Store dry and tightly closed at
+15°C to +25°C. Remove only the number of strips required at the time. Do not touch
reaction zones of the test strips. Tightly close container immediately after removal of test
strip(s).