CAYE Broth Supplement

CAYE Supplement

Cat. No. 1.00051.0001
(Package with 16 vials)

Additive for the preparation of CAYE Broth or modified Brain Heart Infusion Agar for the detection of verotoxins from pathogenic E.coli.

Mode of Action	Experimental Procedure and Evaluation
Typical Composition (g/liter)	Additives/Reagents
Preparation

Mode of Action
CAYE Broth Supplement enhances the production and release of verotoxins produced by pathogenic E.coli during growth.

Composition (per vial)
Verotoxin inducer 10 µg

Preparation
The content is dissolved in the original vial by adding 1 ml of sterile deionized or distilled water.
The content of 1 vial CAYE Broth Supplement is added to 200 ml of CAYE Broth or to 100 ml of Brain Heart Infusion Agar after it has cooled down to about + 45° C.

Experimental Procedure and Evaluation

Broth method
Pick up 1-5 typical colonies from the isolation medium and inoculate a tube with 1ml of the  prepared CAYE Broth with CAYE Broth Supplement.
Incubation:   6 hours at +37°C without stirring.

Agar plate method
Streak one colony onto the surface of a plate containing Brain Heart Infusion Agar with CAYE Broth Supplement.
Incubation:   18-24 hours at  +37°C aerob.

Additives

EMD Cat. No.	Product	Pack Size
1.00060.0100	CAYE Broth mod. acc. to EVANS	100 g
1.13825.0500	Brain Heart Infusion Agar	500 g