Mode of Action
Bolton Selective Enrichment
Broth contains nutrients to aid resuscitation of sublethally damaged cells of Campylobacter. By this
microaerophilic incubation is not needed. Addition of Bolton Broth Selective Supplement inhibits
the accompanying gram-positive and gram-negative bacteria as well as yeasts and molds.
Preparation
Suspend 13.8 g in 500 ml of purified water. Autoclave for 15 min. at 121°C. Cool to 45-50°C.
Aseptically add 25 ml lysed horse blood and the contents of 1 vial of Bolton Selective Supplement.
Mix well and distribute the broth into sterile screw top containers. After the addition of the sample,
the space between the screw top and the surface of the broth should be approximately 2 cm.
pH: 7.4 ± 0.2 at 25°C.
The ready-to-use broth is dark red to black.
Experimental Procedure and Evaluation
Mix 25 g of food saple in 225 ml
Bolton Selective Enrichment Broth and incubate for 4 hours at 37°C. Continue incubation for
an additional 14-44 hours at 41.5°C.
IMPORTANT: During the incubation of the broth, blood particles will form a sediment. This
sediment should not be shaken up when taking out a sample for the inoculation bottle.
Subculture after 18 and 48 hours on Campylobacter Blood-Free Selective Agar.
Direct screening for Campylobacter jejuni and Campylobacter coli using Singlepath
Campylobacter, EMD Cat. No. 1.04143 is possible.
Quality control
Test strains
Growth after 48 hours
Campylobacter jejuni ATCC 33291
> 106 CFU/ml
Campylobacter jejuni ATCC 29428
> 106 CFU/ml
Campylobacter coli ATCC 33559
> 106 CFU/ml
E. coli ATCC 25922
-
Saccharomyces cerevisiae ATCC 9763
-
Additives
EMD Cat. No.
Product
Pack Size
1.00069.0001
Bolton Selective Supplement
16 vials
1.00070.0500
Campylobacter Blood-Free Selective Agar Base (modified CCDA)
