Bolton Supplement


Cat. No. 1.00069.0001
 (Package with 16 vials)
Replaced with Cat. No. 1.00079.0001


Additive for the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.


Mode of Action Preparation
Typical Composition (g/liter) Additives/Reagents


Mode of Action
 Bolton Supplement is a mixture of four different antibiotics in lyophilized form.

Vancomycin, Cefoperazone und Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Cycloheximide largely reduces the growth of yeasts and molds.

Composition (per vial)
 Vancomycin 10 mg; Cefoperazone 10 mg; Trimethoprim 10 mg; Cycloheximide 25 mg

Preparation
 Dissolve the lyophilisate in the original vial by adding of 5 ml of a 50:50 mixture of sterile distilled water and ethanol. Mix well. Complete dissolution will take about 15 minutes; do not proceed until completely dissolved.

Aseptically add contents of 1 vial (5 ml) aseptically to 500 ml of sterile Bolton Enrichment Broth Base cooled to 45-50°C. Mix well.

Additives



EMD Cat. No. Product Pack Size
1.00068.0500 Bolton Selective Enrichment Broth 500 g
N/A Lysed Horse Blood N/A
     







© 2002 Merck KGaA, Darmstadt, Germany