Bolton Broth Selective Supplement

Bolton Supplement

Cat. No. 1.00079.0001
(Package with 16 vials)
Replaces Cat. No. 1.00069.0001

Additive for the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods. The formulation complies with the recommendations of ISO 10272-1.

Mode of Action	Preparation
Typical Composition (g/liter)	Additives/Reagents

Mode of Action
Bolton Supplement is a mixture of four different antibiotics in lyophilized form.

Vancomycin, Cefoperazone und Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of yeasts and molds.

Composition (per vial)
Vancomycin 10 mg; Cefoperazone 10 mg; Trimethoprim 10 mg; Amphotericin B 5 mg

Preparation
Dissolve the lyophilisate in the original vial by adding of 5 ml of a 50:50 mixture of sterile distilled water and ethanol. Mix well. Complete dissolution will take about 15 minutes; do not proceed until completely dissolved.

Aseptically add contents of 1 vial (5 ml) aseptically to 500 ml of sterile Bolton Enrichment Broth Base cooled to 45-50°C. Mix well.

Additives

EMD Cat. No.	Product	Pack Size
1.00068.0500	Bolton Selective Enrichment Broth	500 g
N/A	Lysed Horse Blood	N/A