Tryptic Soy Broth (TSB) non animal origin

Tryptic Soy Broth, Non-Animal Origin

EMD Cat. No. 1.00525.0500/5000

Universal culture medium free from inhibitors and indicators for a wide spectrum of applicaitons. The Peptones used in the broth are from non animal origin. It conforms with the requirements of the pharmaceutical industry and biotechnology.

Mode of Action	Preparation
Experimental Procedure and Evaluation	Quality Control
Typical Composition (g/liter)	Literature

Mode of Action
The medium comply with the recommendations of USP XXIII (1995), EP (1999) and the German Edition of Ph.Eur. (1999). The microbiological performance of the Tryptic Soy Broth (TSB) non animal origin complies with the classical Tryptic Soy Broth (TSB).

Preparation
Suspend 30 g in 1 liter of purified water, autoclave (15 min. at 121x°C).
pH: 7.3 ± 0.2 at 25 °C.
The prepared medium is clear and yellowish-brown.

Experimental Procedure and Evaluation
Depend on the purpose for which the medium is used.
Incubation: 24 hours at 35 °C aerobically, for up to 7 days for the sterility-test at room-temperature.

Quality control (Inoculum approx. 10 100 microorganisms)


Test strains		Growth

Escherichia coli ATCC 8739		good
Staphylococcus aureus ATCC 6538-P		good
Streptococcus pneumoniae ATCC 6301		good
Bacillus subtilis ATCC 6633		good
Pseudomonas aeruginosa ATCC 9027		good
Candida albicans ATCC 10231		good
Candida albicans ATCC 2091		good
Salmonella typhimurium ATCC 14028		good
Aspergillus niger ATCC 16404		good

Typical Composition (g/liter)
Peptone non animal origin 20.0; D(+)-Glucose 2.5; Sodium chloride 5.0; di-potassium hydrogen phosphate 2.5

Literature

Product Brochure

Deutsches Arzneibuch, 10. Auflage; European Pharmacopeia II, United States Pharmacopeia XXIII