LB-Broth (LENNOX)

LB-Broth (LENNOX)

Cat. No. 1.00547.0500/5007
(500 g, 5 kg)

For the cultivation of E. coli in fermentation and molecular genetic studies.

Mode of Action	Experimental Procedure and Evaluation
Typical Composition (g/liter)	Quality Control
Preparation	Literature

Mode of Action
LB-Broth is based on the formulation of LENNOX (1955) supporting growth of E. coli.
Casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Compared to LB-Broth (MILLER) the sodium chloride content is reduced to 5.0 g/l.

Typical Composition (g/liter)
LB-Broth:
Yeast extract 5.0; peptone from casein 10.0; sodium chloride 5.0.

Preparation
Suspend 20 g LB-Broth in 1 liter of purified water and autoclave for 15 min at 121°C.
pH: 7.0 ± 0.2 at 25°C.
The prepared broth is clear and yellowish.

Experimental Procedure
According to appropriate use or purpose.
Incubation: 24 h at 35-37°C aerobically.

Quality control

Test strains	Growth
Escherichia coli ATCC 25922	good
Escherichia coli ATCC 11775	good

Literature

Deaso LENNOX E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190.