TSC Clostridium perfringens Supplement


Cat. No. 1.00888.0001
 (Package with 16 vials)


Additive to TSC Agar Base (Cat. No. 1.11972.0500)



Mode of Action Experimental Procedure and Evaluation
Typical Composition (g/liter) Additives/Reagents
Preparation


Mode of Action
 D-Cycloserine inhibits the accompanying bacterial flora and causes the colonies which develop to remain smaller. It also reduces a diffuse and thus disturbing blackening around the Clostridium perfringens colonies. 4-Methylumbelliferylphos- phate (MUP) is a fluorogenic substrate for the alcaline and acid phosphatase. The acid phopshatease is a high specific indicator for Clostridium perfringens.
 The acid phosphatase splits the fluorogenic substrate MUP forming 4-methylumbelliferone which can be identified as it fluorescence in long wave UV light. Thus a strong suggestion for the presence of Clostridium perfringens can be obtained.

Typical Composition
 200 mg D-Cycloserine; 50 mg 4-methylumbelliferyl-phosphate disodium salt.

Preparation
 Add 5 ml of sterile purified water to 1 vial and dissolve the mixture. To prepare 500 ml of TSC Agar, add the dissolved mixture to the sterile culture medium base cooled to a temperature of 50°C. Mix the supplement homogeneously into the culture medium by carefully swirling.
 pH of the ready-to-use medium: 7.4 ± 0.2 at 25°C.
 The ready plates (incl. supplelment) are clear and light brown.

Experimental Procedure and Evaluation
 Inoculate by the pour plate technique only.
 Incubate: 18-24 hours at 44°C under anaerobic conditions (e.g. Anaerocult® A, Anaerocult® A mini).
Fluorescence can be detected with an UV lamp; light blue fluorescencing black colonies indicate Clostridium perfringens.
 For Quality control please refer to TSC Agar Base (Cat. No. 1.11972).

Additives



EMD Cat. No. Product Pack Size
1.10641.0007 Anaerocult® A mini 1 x 25
1.13203.0001 UV Lamp (366 nm) 1 ea
13677 Anaerocult® A 1 x 10
     







© 2002 Merck KGaA, Darmstadt, Germany