Readycult® Enterococci

EMD Cat. No. 1.01299.0001

20 tests

Content: 20 snap packs
1 snap pack each for 100 ml of water sample.

Application
Selective enrichment broth for the detection of Enterococci and D-Streptococci within the bacteriological water examination.

Principle
The peptone mixture guarantees rapid growth of Enterococci. Sodium-azide largely inhibits the accompanying flora, especially the Gram-negative.
The chromogenic substrate X-GLU is cleaved, stimulated by selected peptones, by the enzyme -D-Glucosidase present in Enterococci. This results in an intensive blue-green color change of the broth.

Additives

EMD Cat. No.	Product	Pack Size
1.07551.2000	EXTRAN® MA 04 disinfectant	2 liter

Experimental Procedure and Evaluation	Quality Control
Typical Composition (g/liter)	Picture/Image

Procedure

1.	Add 100 ml of water sample into a sterile, transparent 250 ml vessel with screw cap. In case the sample is to be stored below +25 °C, the examination has to be started within 6 hours. Exceptionally the sample can be stored at +2 to +8 °C (refrigeration) for up to 24 hours.
2.	Take one snap pack, shortly tap to ensure the granules are at the bottom. Bend the upper part of the snap pack until it breaks open. Attention: do not touch the opening to avoid contamination risk!
3.	Add the contents to the water sample. Seal the vessel and shake to dissolve the granules completely.
4.	Incubation: 18-24 h at 35 to 37 °C. If incubated at room temperature (+20 to +25 °C) the incubation time is prolonged to 48 hours.

Interpretation of Results
Negative: No color change.
The broth remains slightly yellow.

Positive: Any color change of the broth to blue-green, even in the upper section of the broth only, confirms the presence of Enterococci (X-GLU reaction).
No decoloration with shaking!

Disposal
Autoclave the broth (15 min./121 °C). Alternatively heat the broth for 30 min. in boiling water or use a proper disinfectant (e.g. Extran^® MA 04).

Storage
Store dry at +15 °C to +25 °C.

Shelf life
If stored under recommended conditions the unopened snap pack has a shelf-life of 3 years after day of production (see expiry date on the label).

Quality control

 

Test strains	Growth	color change to blue-green
Enterococcus faecalis ATCC 19433	fair / good	+
Staphylococcus aureus ATCC 25923	fair / good	-

Composition in g/snap pack
Peptones 0.86; sodium chloride 0.64; sodium-azide 0.06; X-GLU 0.004; Tween^® 80 0.22.
pH: 7.5 ± 0.2 at 25 °C.

                
                  Readycult Enterococci                                                                        Enterococcus faecalis