Alkaline Peptone Water


Cat. No. 1.01800.0500
 (500 g)

For the enrichment of Vibrio cholera and Vibrio spp. in food and other materials.

 

Mode of Action Typical Composition
Preparation Experimental Procedure and Evaluation
Quality Control Additives
Literature  


Mode of Action
 The medium complies with the recommendation of FDA/BAM, 8th Edition 1995, Chapter 9: Vibrio cholera, V.parahamolyticus, V.vulnificus and other Vibrio spp.; American Public Health Association (APHA), Chapter 28: VIBRIO; AOAC Official Method 988.20 Detection of Vibrio cholera in Oysters; ISO 8914, 1990 Detection of Vibrio parahaemoloyticus.

The growth of a broad spectrum of Vibrio spp. is promoted using peptones, a sodium chloride concentration of 10 g/liter and a pH of 8.5.

Typical Composition (g/liter)
 Peptone 10.0; Sodium Chloride 10.0.

Preparation
 Suspend 20 g in 1 liter of purified water. Autoclave (15 min at 121°C).

pH: 8.5 + 0.2 at 25°C.

The prepared medium is clear and yellowish brown.

Experimental Procedure and Evaluation
 Inoculate Alkaline Peptone Water (usually add 25 g of saple material to 225 ml of broth); incubate 6-8 h and 16-24 h at 35-37°C.

After 6-8 h and 16-24 h of incubation, streak 0.1 ml on the surface of TCBS Agar (Cat. No. 1.10263) so that single colonies are well isolated.

Additional tests for the differentiation and identification of Vibrio spp. to follow as described in the standards.

Quality control

Test strains
 Growth
Vibrio vulnificus ATCC 33149
 > 106 c.f.u./ml
Vibrio cholerae eltor Inaba CH 38
 > 106 c.f.u./ml
Vibrio cholerae eltor Ogawa CH 60
 > 106 c.f.u./ml
Vibrio parahaemolyticus ATCC 17802
 > 106 c.f.u./ml


Additives

EMD Cat. No. Product Pack Size
1.10263.0500 TCBS Agar 500 g
1.10270.0500 DCLS Agar 500 g
     
 
Literature
FDA-BAM, 8th Edition 1995, Chapter 9: Vibrio cholera, V. parahaemolyticus, V. vulnificus and other Vibrio spp. American Public Health Association (APHA), Chapter 28: VIBRIO; AOAC Official Method 988.20 Detection of Vibrio cholera in Oysters.

ISO 8914, 1990 Detection of Vibrio parahaemoloyticus.

© 2002 Merck KGaA, Darmstadt, Germany