Campylobacter Agar Base


Cat. No. 1.02248.0500
 (500 g)


Medium proposed by SKIRROW (1977) for the isolation of Campylobacter from clinical material in human and veterinary medicine as well as from contaminated foodstuffs, water etc.



Campylobacter fetus is the causative agent of enzootic abortions and enteritis in domestic livestock (MÃœLLER 1980). Campylobacter jejuni and Campylobacter coli in particular are largely responsible for the Campylobacter enteritis which affects humans (SKIRROW 1977, BUTZLER and SKIRROW 1979, BOKKENHEUSER et al. 1979, BLASER et al 1980). In man, Campylobacter is most commonly transmitted by foodstuffs derived from infected animals, water or direct contact with infected animals (ROBINSON et al. 1982, STERN and KOTULA 1982, CHRISTOPHER et al. 1983).

Mode of Action Quality Control
Typical Composition (g/liter) Additives/Reagents
Preparation Literature
Experimental Procedure and Evaluation


Mode of Action
 A nutrient-rich culture medium and an O2-deficient, CO2-enriched atmosphere ensure that Campylobacter grows well. The antibiotics which are added as a Campylobacter selective supplement largely inhibit the accompanying microbial flora.

Typical Composition (g/liter)
 Peptone-protein mixture 21.0; electrolyte 5.0; starch, soluble 1.0; agar-agar 13.0.
 Also to be added:
 blood 50-70 ml; Campylobacter Selective Supplement 5 vials.

Preparation
 Suspend 40 g in 1 liter of purified water. Autoclave (15 min at 121°C), cool to 40-50°C, add 5-7 % defibrinated blood (sheep, horse) and mix in 1 vial of Campylobacter Selective Supplement per 200 ml culture medium, pour plates.
 pH: 7.3 ± 0.2 at 25°C.
 Before adding blood, the prepared medium is clear and yellowish-brown; afterwards light red and non-hemolytic.

Experimental Procedure and Evaluation
 Inoculate by spreading the sample material on the surface of the plates.
 Incubation: 24-48 hours in an O2-deficient, CO2-enriched atmosphere which can be produced in an anaerobic jar with the aid of Anaerocult® C or in the special incubation bag with the aid of Anaerocult® C mini. The Campylobacter species can be classified, to some extend, according to the dependence of their growth at different temperatures (see Table).

Campylobacter species
 		Incubation temperature

25°C
 		37°C
 		42°C
C. fetus ssp. fetus
 		+
 		+
 		-
C. jejuni/coli
 		-
 		+
 		+
C. fetus spp. venerealis
 		+
 		+
 		-


Quality control


Test strains
 Growth
 		Clear zone
Campylobacter jejuni ATCC 33560
 		(42°C)
 		good / very good
Campylobacter fetus ATCC 27374
 		(35°C)
 		good / very good
Campylobacter coli ATCC 43478
 		(42°C)
 		good / very good
Escherichia coli ATCC 25922
 		(42°C)
 		none / poor
Enterobacter cloacae ATCC 13047
 		(42°C)
 		none / poor
Proteus mirabilis ATCC  29906
 		(42°C)
 		none / poor


Additives



EMD Cat. No. Product Pack Size
1.02249.0001 Campylobacter Selective Supplement 16 vials
13674 Plate basket 1ea
13699 Anaerocult® C mini 1 x 25
14255 Anaeroclip® 1 x 25
13678 Anaerocult® C 1 x 10
13681 Anaerobic jar 1 ea
  Defibrinated sheep or horse blood  
     


Literature

BLASER, M.J., LAFORCE, F.M., WILSON, N.A., a. WANG, W.-LL.: Reservoirs for human campylobacteriosis. - J. Infect. Diseases, 141; 665-669 (1980).
 BOKKENHEUSER, V.D., RICHARDSON, N.J., BRYNER, J.H., ROUX, D.J., SCHUTTE, A.B., KOORNHOF, H.J., FREIMAN, I., a. HARTMAN, E.: Detection of enteric campylobacteriosis in children. - J. Clin. Microbiol., 9; 227-232 (1979).
 BUTZLER, J.P., a. SKIRROW, M.S.: Campylobacter enteritis. - Clin. Gastroenterol., 8; 737-765 (1979).
 CHRISTOPHER, F.M., SMITH, G.C., a. VANDERZANT, C.: Examination of poultry giblets, raw mild and meat for Campylobacter fetus susp. jejuni. - Archiv Lebensmittelhyg., 33; 151 (1982).
 HERBERT, G.A., HOLLIS, D.G., WEAVER, R.E., LAMBERT, M.A., BLASER, M.J., a. MOSS, C.W.: 30 Years of Campylobacter: Biochemical characteristics a biotyping proposal for Campylobacter jejuni. - J. Clin. Microbiol., 15; 1065-1073 (1983).
 MÃœLLER, H.E.: Campylobacter fetus-infektionen - eine Ãœbersicht. - Hyg. + Med., 5; 26-30 (1980).
 ROBINSON, D.A., a. JONES, D.M.: Milkborne Campylobacter infection. - Brit. Med. J., 282; 1374-1377 (1981).
 SKIRROW, M.B.: Campylobacter enteritis: a "new" disease. - Brit. Med., 2; 9-11 (1977).
 STERN, M.J., a. KOTULA, A.W.: Survival of Campylobacter jejuni inoculated into Ground Beef. - Appl. Envirnm. Microbiol., 44; 1150-1153 (1982).
 VERON, M., a. CHATELAIN, R.: Taxonomic Study of the Genus Campylobacter Sebald and Véron and Designation of the Neotype Stain for the Type Species Campylobacter fetus (Smith and Taylor) Sebald and Véron. - Int. J. Bacteriol., 23; 122-134 (1973).


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