Yeast Extract Agar

Yeast Extract Agar

EMD Cat. No. 1.03750.0500

500 g

For the cultivation of yeasts and molds from various materials, especially from milk and dairy products.

Preparation	Experimental Procedure and Evaluation
Quality Control	Typical Composition (g/liter)

Preparation
Suspend 35 g/liter, autoclave (15 min at 121 °C).
pH: 6,5 ± 0.2 at 25 °C.
The plates are clear and yellowish.

Experimental Procedure and Evaluation
Inoculate the culture medium by the pour-plate method or by spreading the material on the surface. The subsequent procedure depends on the purpose for which the medium is used.
Incubation: up to 7 days at 28 °C aerobically.

Quality control

Test strains	Growth
Staphylococcus aureus ATCC 25923	good / very good
Escherichia coli ATCC 25922	good / very good
Candida albicans ATCC 10231	good / very good
Geotrichum candidum DSM 1240	good / very good
Aspergillus niger	good / very good
Penicillium spp.	good / very good
Rhodotorula mucilaginosa DSM 70403	good / very good

Typical Composition (g/liter)
Yeast extract 5.0; glucose 10.0; agar-agar 20.0.