KLIGLER Agar (Double sugar iron agar acc. to KLIGLER)

KLIGLER Iron Agar

Cat. No. 1.03913.0500
(500 g)

Test culture medium proposed by KLIGLER (1917, 1918) for identifying Gram-negative intestinal bacteria.

KLIGLER agar can be modified as proposed by BADER and HOTZ (1951) by adding 0.2 % urea to give iron-urea agar.

Mode of Action	Quality Control
Typical Composition (g/liter)	Additives/Reagents
Preparation	Literature
Experimental Procedure and Evaluation

Mode of Action
See Triple Sugar Iron Agar.

Typical Composition (g/liter)
Peptone from casein 15.0; peptone from meat 5.0; meat extract 3.0; yeast extract 3.0; sodium chloride 5.0; lactose 10.0; D(+)glucose 1.0; ammonium iron(III) citrate 0.5; sodium thiosulfate 0.5; phenol red 0.024; agar-agar 12.0.

Preparation
Suspend 55 g/liter, dispense into test tubes, autoclave (15 min at 121°C). Allow to solidify to give sugar slants.
pH: 7.4 ± 0.2 at 25°C.
The plates are clear and red.

Experimental Procedure and Evaluation
See Triple Sugar Iron Agar.

Quality control

Test strains	Growth	Butt	Slant
Escherichia coli ATCC 25922	good / very good	yellow	yellow
Citrobacter freundii ATCC 8090	good / very good	yellow and black	yellow
Enterobacter cloacae ATCC 13047	good / very good	yellow	yellow
Shigella flexneri ATCC 12022	good / very good	yellow	red
Salmonella typhimurium ATCC 14028	good / very good	yellow and black	red
Salmonella enteritidis ATCC 13076	good / very good	yellow and black	red
Proteus mirabilis ATCC 14153	good / very good	yellow	red
Proteus vulgaris ATCC 13315	good / very good	yellow and black	red

Additives

EMD Cat. No.	Product	Pack Size
9510	Urea	500 g

Literature

BADER, R.E., u. HOTZ, G.: Eisen-Harnstoff-Agar, eine Modifikation des Eisen-Agars nach KLIGLER. - Z. Hyg. Infekt.-Kr., 133; 20-25 (1951).
KLIGLER, I.J.: A simple medium for the differentiation of members of typhoid-paratyphoid group. - Am. J. Publ. Health, 7; 1042-1044 (1917).
KLIGLER, I.J.: Modification of culture media used in the isolation and differentiation of typhoid, dysentery and allied bacilli. - J. Exper. Med., 28; 318-322 (1918).