Fluorocult MacCONKEY Agar

Fluorocult^® MacCONKEY Agar

EMD Cat. No. 1.04029.0500

500 g

Additives

EMD Cat. No.	Product	Pack Size
1.13203.0001	UV Lamp (366 nm)	1 ea

Typical Composition (g/liter)

Quality Control

Mode of Action
The culture medium serves to isolate Salmonella, Shigella and coliform bacteria, in particular Escherichia coli, from various materials. The bile salts and crystal violet largely inhibit the growth of Gram-positive microbial flora. Lactose together with the pH indicator neutral red are used to detect lactose-positive colonies and E. coli can be seen among these because of fluorescence under UV light.

Preparation
Suspend 50.1 g/liter, autoclave (15 min at 121 °C) pour plates.
pH: 7.1 ± 0.2 at 25 °C.
The plates are clear and red to red-brown.

Experimental Procedure and Evaluation
Inoculate plates in the usual way and incubate for 18 to 24 hours at 35 °C aerobically.
Lactose-negative colonies are colorless. Lactose-positive colonies are red and often surrounded by a turbid zone due to the precipitation of bile acids.
Inspect the culture with UV light: light blue fluorescence denotes E. coli.

Quality control

Test strains	Growth	color of		Precipitate	MUG
		colony	medium
Escherichia coli ATCC 11775	good / very good	red	red	+
Escherichia coli ATCC 25922	good / very good	red	red	+	+
Enterobacter aerogenes ATCC 13048	good / very good	red	red	-	-
Salmonella typhimurium ATCC 13311	good / very good	colorless	yellowish	-
Salmonella dublin ATCC 15480	good / very good	colorless	yellowish	-
Shigella sonnei ATCC 11060	good / very good	colorless	yellowish	-
Proteus mirabilis ATCC 29906	good / very good	colorless	yellowish	-
Bacillus cereus ATCC 11778	none
Staphylococcus aureus ATCC 6538-P	none
Enterococcus hirae ATCC 8043	none

Typical Composition (g/liter)
Peptone from casein 17.0; peptone from meat 3.0; sodium chloride 5.0; lactose 10.0; bile salt mixture 1.5; neutral red 0.03; crystal violet 0.001; agar-agar 13.5; 4-methylumbelliferyl-β-D- glucuronide 0.1.