TBG Broth Modified

EMD Cat. No. 1.05178.0500
500 g


Selective enrichment of Salmonella for the examination of pharmaceutical products in raw materials, foodstuffs, meat etc.

The broth complies with the recommendations of the German DAB 10 and the European Pharmacopeia II.
Note: for granting good functioning of the TBG-Broth, the preparation and the incubation temperature had to be modified versus the recommendations of DAB 10/EP.


Additives


EMD Cat. No. Product Pack Size
1.02896.0500 LEIFSON Agar 500 g
1.05287.0500 XLD Agar 500 g
1.07232.0500 Brilliant Green Phenol Red Lactose Agar (USP) 500 g
1.07661.0500 Lactose Broth 500 g



Mode of Action Preparation
Experimental Procedure and Evaluation Quality Control
Typical Composition (g/liter) Literature


Mode of Action
 Bile supports the growth of enteric bacteria and inhibits bacteria, which do not normally live in the intestine.
Brilliant-green specifically inhibits the Gram-positive accompanying flora. For suppressing Proteus, the pH of the medium can be adjusted to approx. 6.5. JEFFRIES (1959) reported that it is advantageous to add 0.04 g/liter novobiocin for suppressing Proteus.
Preparation
 Suspend 63 g in 1 liter of purified water, if needed short heating to a maximum of 50 °C. Any undissolved calcium carbonate should be homogenously mixed before pouring.
  •   Do not autoclave!
  •   adjust pH to a value of 7.0 ± 0.2.
     The preparared broth is turbid, green with white sediment.
    Experimental Procedure and Evaluation
    1.
         		Sample to be enriched in Lactose-Broth.
    2.
         		From the pre-enrichment inoculate an appropriate amount into TBG-Broth and incubate for 18-24 h at 35-37 °C.
    3.
         		Streak onto appropriate Salmonella media.
    4.
         		Brown cultures to be examined further.

    Quality control



    		Growth
         		after
    Test strains
    		 Inoculum
         		6 h
         		24 h
    Escherichia coli ATCC 25922
         		approx. 99 %
         		<= 30 %
         		<= 5 %
    Salmonella typhimurium ATCC 14028
         		approx. 1 %
         		<= 70 %
         		<= 95 %

    Typical Composition (g/liter)
     Peptone 8.6; ox-bile 8.0; sodium chloride 6.4; calcium carbonate 20.0; potassium tetrathionate 20.0; brilliant green 0.07.
    Literature

    Deutsches Arzneibuch (DAB), 10. Auflage, Kapitel VIII, 10.
     European Pharmacopeia II, Kapitel VIII, 10
     JEFFRIES, L.: Novobiocin-tetrathionate broth: A medium of improved selectivity for the isolation of salmonellae from faeces. - J. Clin. Path., 12; 568-571 (1959)




    © 2002 Merck KGaA, Darmstadt, Germany