EE Broth, MOSSEL

EMD Cat. No. 1.05394.0500/5000
500 g, 5 kg


Medium proposed by MOSSEL et al. (1963, 1964) for the selective enrichment of all species of Enterobacteriaceae from foodstuffs and other materials.

This medium complies with the specifications of the Eiprodukte-Verordnung (German Egg Product Regulations) (1975) and the European Pharmacopeia II.

Mode of Action Preparation
Experimental Procedure and Evaluation Quality Control
Typical Composition (g/liter) Literature
Picture/Image


Mode of Action
 The undesired, accompanying bacterial flora is almost completely inhibited by brilliant green and ox bile. Dextrose favours the growth of all Enterobacteriaceae. The strong buffering capacity of the culture medium prevents the formed acid from killing the culture.

Typical Composition (g/liter)
 Peptones 10.0; D(+)glucose 5.0; ox bile, dried 20.0; brilliant green 0.0135; di-sodium hydrogen phosphate-dihydrate 8.0; potassium dihydrogen phosphate 2.0.

Preparation
 Suspend 45 g in 1 liter of purified water. Dispense into test tubes and autoclave under mild conditions (5 min at 121°C), or heat at 100°C for 30xminutes in a waterbath or flowing steam.
 pH: 7.2 ± 0.2 at 25°C.
 The prepared broth is clear and green.

Experimental Procedure and Evaluation
 Inoculate the broth with the sample material.
 Incubation: 24-48 hours at 35°C aerobically.
If the medium shows bacterial growth, transfer some material to selective culture media for identification.

Quality control

Test strains
 Inoculum (cfu)
 		Growth
Escherichia coli ATCC 8739
 		10-100
 		good
Escherichia coli ATCC 25922
 		10-100
 		good
Salmonella typhimurium ATCC 14028
 		10-100
 		good
Staphylococcus aureus ATCC 6538
 		> 103
 		inhibited
Micrococcus luteus ATCC 10240
 		> 103
 		inhibited
Bacillus cereus ATCC 11778
 		> 103
 		inhibited
Enterococcus faecalis ATCC 19433
 		> 103
 		inhibited


Literature

Bundesminister für Jugend, Familie und Gesundheit: Verordnung über die gesundheitlichen Anforderungen an Eiprodukte und deren Kennzeichnung (Eiprodukte-Verordnung) (1975).
 European Pharmacopeia II. Chaptre VIII. 10.
 MOSSEL, D.A.A., u. MARTIN, G.: Eine mit dem Salmonella-Nachweis kommensurable Untersuchung von Lebens- und Futtermitteln auf Enterobacteriaceae. - Arch. f. Lebensmittelhyg., 15; 169-171 (1964).
 MOSSEL, D.A.A., MENGERINCK, W.J.H., a. SCHOLTS, H.H.: Use of a modified MacConkey agar medium for the selective growth and enumeration of all Enterobacteriaaceae. - J. Bact., 84; 381 (1962).
 MOSSEL, D.A.A., VISSER, M., a. CORNELISSEN, A.M.R.: The examination of foods for Enterobacteriaceae using a test of the type generally adapted for the detection of salmonellae. - J. Appl. Bact., 24; 444-452 (1963).


All test strains as listed in the Quality control table

Enterobacteriaceae Enrichment Broth acc. to Mossel


© 2002 Merck KGaA, Darmstadt, Germany