ROGOSA Agar


Cat. No. 1.05413.0500
 (500 g)


Medium proposed by ROGOSA, MITCHELL and WISEMANN (1951) for the isolation and enumeration of lactobacilli in the oral and intestinal microbial flora, meat, milk and other foodstuffs.



Mode of Action Quality Control
Typical Composition (g/liter) Additives/Reagents
Preparation Picture/Image
Experimental Procedure and Evaluation Literature


Mode of Action
 The accompanying bacterial flora is largely suppressed by the high acetate concentration and the low pH value. Low concentrations of manganese, magnesium and iron ensure optimal growth of lactobacilli.


Typical Composition (g/liter)
 Peptone from casein 10.0; yeast extract 5.0; D(+)glucose 20.0; potassium dihydrogen phosphate 6.0; ammonium citrate 2.0; Tween® 80 1.0; sodium acetate 15.0; magnesium sulfate 0.575; iron(II) sulfate 0.034; manganese sulfate 0.12; agar-agar 15.0.

Preparation
 Suspend 74.5 g/liter, adjust the pH to 5.5 with acetic acid 96 % (approx. 1.3 ml/liter).

n  Do not autoclave.
 pH: 5.5 ± 0.2 at 25°C.
 The plates are clear and yellowish-brown.

Experimental Procedure
 Inoculate by the pour-plate technique or by spreading the material on the surface of the culture medium.
 Incubation: up to 3 days at 35°C or 5 days at 30°C (SHARPE 1960) under anaerobic conditions in a 5 % carbon dioxide atmosphere.
 Establish the bacterial count. For the purpose of identification, reinoculate individual colonies and subject them to the necessary tests (MITSUOKA 1969).


Quality control (spiral plating method)


Test strains
 Inoculum (cfu/ml)
 		Recovery rate %
Lactobacillus acidophilus ATCC 4356
 		103-105
 		≥ 70
Lactobacillus casei ATCC 393
 		103-105
 		≥ 70
Lactobacillus fermentum ATCC 9338
 		103-105
 		≥ 70
Lactobacillus plantarum ATCC 8014
 		103-105
 		≥ 70
Bifidobacterium bifidum ATCC 11863
 		103-105
 		≥ 70 (anaerobic)
Escherichia coli ATCC 11755
 		> 105
 		£0.01
Proteus vulgaris ATCC 13315
 		> 105
 		£0.01
Enterococcus faecalis ATCC 11700
 		103-105
 		£0.01


Additives



EMD Cat. No. Product Pack Size
B10001-74 Acetic acid min. 96 % 500 ml
     



Lactobacillus Plantarum ATCC 8014


Rogosa Agar - Lactobacillus acidophilus

Literature

MITSUOKA, T.: Vergleichende Untersuchungen über Lactobazillen aus den Faeces von Menschen, Schweinen und Hühnern. – Zbl. Bakt. I. Orig., 210; 32-51 (1969).
 ROGOSA, M.; MITCHEL, J.A., a. WISEMAN, R.F.: A selective medium for the isolation of oral und faecal lactobacilli. – J. Bact. 62; 132-133 (1951).
 SHARPE, M.E.: Selective media for the isolation and enumeration of lactobacilli. – Lab. Practice, 9; 223-227 (1960).


© 2002 Merck KGaA, Darmstadt, Germany