SABOURAUD-4 % Maltose Agar

SABOURAUD 4%-Maltose Agar

EMD Cat. No. 1.05439.0500

500 g

This culture medium complies with the recommendations of the United States Pharmacopeia XXII (1995) and the European Pharmacopeia II.

Preparation	Experimental Procedure and Evaluation
Quality Control	Typical Composition (g/liter)
Literature	Picture/Image

Preparation
Suspend 65 g/liter, autoclave (15 min at 121°C).
Do not overheat.
pH: 5.6 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.

Experimental Procedure and Evaluation
The plates are inoculated with sample material according to the instructions. The fungi colonies which have grown are judged macro- and microscopically.
Incubation: up to 7 days at 28°C aerobically.

Quality control

Test strains	Growth (1.05438.)	Growth (1.05439.)
Trichophyton mentagrophytes	fair / very good	good / very good
Trichophyton rubrum	fair / good	fair / good
Microsporum gallinae ATCC 12108	fair / very good	good / very good
Trichophyton ajelloi	fair / good	fair / good
Microsporum canis	good / very good	good / very good
Geotricum candidum DSM 1240	good / very good	good / very good
Aspergillus niger	good / very good	good / very good
Penicillium commune ATCC 10428	good / very good	fair / good

Quality control (spiral plating method)

Test strains	inoculum (cfu/ml)	Recovery rate %
Candida albicans ATCC 10231	10³-10⁵	>= 70
Candida albicans ATCC 2091	10³-10⁵	>= 70

Typical Composition (g/liter)
Peptone 10.0; maltose 40.0; agar-agar 15.0.

Literature

European Pharmacopeia II, Chapter VIII, 10.
United States Pharmacopeia XXIII, Chapter "Microbial Limit Tests" (1995).

Candida albicans