Anaerobic Agar, BREWER

EMD Cat. No. 1.05452.0500
500 g


For the surface cultivation of clostridia and other anaerobic microorganisms according to BREWER (1940, 1942).
Experimental Procedure and Evaluation Quality Control
Typical Composition (g/liter) Literature


Mode of Action
 The medium contains a series of reducing agents (thioglycollate, formaldehydesulfoxylate, cystine) which ensure adequate anaerobiosis (QUASTEL and STEPHENSON 1926, AUBERTIN et al. 1928). Methylene blue serves as a redox indicator, its decoloration indicates anaerobiosis.
Typical Composition (g/liter)
 Peptone from casein 10.0; peptone from soymeal 5.0; yeast extract 5.0; L-cystine 0.4; D(+)glucose 10.0; sodium chloride 5.0; sodium thioglycollate 2.0; sodium formaldehydesulfoxylate 1.0; methylene blue 0.002; agar-agar 12.6.
Preparation
 Suspend 51 g in 1 liter of purified water. Autoclave (15 min at 121°C), pour plates to give thick layers.
pH: 7.2 ± 0.2 at 25 °C.
 The plates are clear and light green.
Experimental Procedure and Evaluation
 Inoculate the culture medium using pour-plate method. For the identification of spore-forming microorganisms add the sample material at a temperature of 80-100 °C.
 Incubation: incubate up to 48 hours at 35 °C in an anaerobic atmosphere under optimal conditions (e.g. with Anaerocult® A, Anaerocult® P or Anaerocult® A mini).
Quality control

Test strains
 		Growth
Clostridium tetani ATCC 19406
 		fair
Clostridium botulinum
 		good/very good
Clostridium perfringens ATCC 10543
 		good/very good
Clostridium putrificum ATCC 25784
 		good/very good
Clostridium septicum ATCC 12464
 		good/very good
Clostridium novyi 1795
 		good/very good
Staphylococcus aureus ATCC 25923
 		medium/very good
Escherichia coli ATCC 25922
 		good/very good

Additives

EMD Cat. No. Product Pack Size
1.10641.0007 Anaerocult® A mini 1 x 25
13674 Plate basket 1ea
Anaerocult® P 1 x 25
13677 Anaerocult® A 1 x 10
14255 Anaeroclip® 1 x 25
1.13675 Anaerotest® 1 x 50
13681 Anaerobic jar 1 ea


Literature

AUBERTIN, E., AUBEL, E., et GENEVOIS, L.: A propos de la culture des anaérobies strict en milieu, aérobie. - Compt. rend. Soc. Biol. (PARIS), 98; 957-959 (1928).
 BREWER, J.H.: Clear liquid medium for the "aerobic" cultivation of anaerobes. - .J. Amer. Med. Ass., 115; 598-600 (1940).
 BREWER, J.H.: A new Petri dish and technique for use in the cultivation of anaerobes and microaerophiles. - Science, 95; 587 (1942).
 QUASTEL, J.H., a STEPHENSON, M.: Experiments on "strict" anaerobes: I. The relationship of B. sporogenes to oxygen . - Biochem. J., 20; 1125-1137 (1926).