Tryptic Soy Agar

Tryptic Soy Agar

EMD Cat. No. 1.05458.0500/5007

500 g, 5 kg

Universal culture media free from inhibitors and indicators for a wide spectrum of applications.

They comply with the recommendations of the United States Pharmacopeia 30 and the European Pharmacopeia 6.

Click here for information about harmonization of USP/EP chapters on Microbiological Examination of Non-sterile Products (formerly called Microbial Limits Test).

Typical Composition (g/liter) Literature

Typical Composition (g/liter)
Peptone from casein 15.0; peptone from soymeal 5.0; sodium chloride 5.0; agar-agar 15.0.

Preparation
Suspend 40 g/liter (agar) or 30 g/liter (broth), autoclave (15 min at 121 °C).
pH: 7.3 ± 0.2 at 25 °C.
After preparation both media are clear and yellowish-brown.

Experimental Procedure and Evaluation
Depend on the purpose for which the media are used.
Incubation: 24 hours at 35 °C aerobically, for up to 7 days for the sterility-test at room-temperature.

Quality control (spiral plating method)

Test strains	Inoculum (cfu/ml)	Recovery rate (%)
Escherichia coli ATCC 8739	10 - 100	≥70
Staphylococcus aureus ATCC 6538	10 - 100	≥70
Streptococcus pyogenes ATCC 21059	10 - 100	≥70
Bacillus subtilis ATCC 6633	10 - 100	≥70
Candida albicans ATCC 10231	10 - 100	≥70
Pseudomonas aeruginosa ATCC 9027	10 - 100	≥70
Aspergillus niger ATCC 16404	10 - 100	≥50

Literature

HAWKEY, P.H., MCCORMICK, A., a. SIMPSON, R.A.: Selective and differential medium for the primary isolation of members of the proteae. J. Clin. Microbiol. 23; 600-603 (1986).

United States Pharmacopeia 30

<61> Microbiological Examination of Non-sterile Products: Microbial Enumeration Tests (USP 30, page 89).
<62> Microbiological Examination of Non-sterile Products: Tests for Specified Microorganisms (USP 30, page 93).

European Pharmacopeia 6 (Ph. Eur)
2.6.12 Microbiological Harmonization of Non-sterile Products - Microbial Enumeration Test
2.6.12 Microbiological Harmonization of Non-sterile Products - Tests for Specified Microorganisms