Tryptic Soy Broth

EMD Cat. No. 1.05459.0500/5007/9010/9027
(500 g, 5 kg, 10 kg, 5 kg)


Universal culture media free from inhibitors and indicators for a wide spectrum of applications.

They comply with the recommendations of the United States Pharmacopeia 30 and the European Pharmacopeia 6.

Click here for information about harmonization of USP/EP chapters on Microbiological Examination of Non-sterile Products (formerly called Microbial Limits Test).


Preparation Experimental Procedure and Evaluation
Quality Control Typical Composition (g/liter)
Literature


Typical Composition (g/liter)
 Peptone from casein 15.0; peptone from soymeal 5.0; sodium chloride 5.0; agar-agar 15.0.
Preparation
 Suspend 40 g/liter (agar) or 30 g/liter (broth), autoclave (15 min at 121 °C).
pH: 7.3 ± 0.2 at 25 °C.
 After preparation both media are clear and yellowish-brown.
Experimental Procedure and Evaluation
 Depend on the purpose for which the media are used.
 Incubation: 24 hours at 35 °C aerobically, for up to 7 days for the sterility-test at room-temperature.


Quality control

Test strains
 Inoculum
 Growth
24 hrs at 35°C
 Growth
3 Days at 20-25°C
 Growth
5 Days at 20-25°C
Escheria coli ATCC 8739
 10 - 100
 +
  
  
Staphylococcus aureus ATCC 6538
 10 - 100
 +
 +
  
Streptococcus pneumoniae ATCC 6301
 10 - 100
 +
  
  
Bacillus subtilis ATCC 6633
 10 - 100
 +
 +
  
Candida albicans ATCC 10231
 10 - 100
  
  
 +
Candida albicans ATCC 2091
 10 - 100
  
  
 +
Pseudomonas aeruginosa ATCC 9027
 10 - 100
 +
  
  
Aspergillus niger ATCC 16404
 10 - 100
  
  
 +
Staphyloccus aureus 25923
 10 - 100
  
 +
  
Salmonella typhinurium ATCC 14028
 10 - 100
 +
  
  


Literature

Product Brochure

 HAWKEY, P.H., MCCORMICK, A., a. SIMPSON, R.A.: Selective and differential medium for the primary isolation of members of the proteae. J. Clin. Microbiol. 23; 600-603 (1986).

United States Pharmacopeia 30

<61> Microbiological Examination of Non-sterile Products: Microbial Enumeration Tests (USP 30, page 89).
<62> Microbiological Examination of Non-sterile Products: Tests for Specified Microorganisms (USP 30, page 93).

European Pharmacopeia 6 (Ph. Eur)
2.6.12 Microbiological Harmonization of Non-sterile Products - Microbial Enumeration Test
2.6.12 Microbiological Harmonization of Non-sterile Products - Tests for Specified Microorganisms






© 2002 Merck KGaA, Darmstadt, Germany