OGYE Agar Base


Cat. No. 1.05978.0500
 (500 g)


Medium for the selective isolation and enumeration of yeast and molds in food.


Oxytetracycline-Glucose-Yeast Agar (OGYE Agar) described by MOSSEL et al. (1962, 1970) is used for the isolation and enumeration of yeast and molds in foods.
Mode of Action Typical Composition
Preparation Quality Control
Additives/Reagents Literature
Experimental Procedure and Evaluation Picture/Image


Mode of Action
 The base medium allows good growth of yeast and molds. Oxytetracycline inhibits the growth of bacteria.

Typical Composition (g/liter)
 Yeast Extract 5.0; D(+)glucose (dextrose) 20.0; Agar-Agar 12.0

Preparation
Suspend 18.5 g in 500 ml of purified water. Heat to boiling to dissolve completely. Autoclave at 121°C fr 15 minutes. Cool the medium to 45-50°C and aseptically add the contents of 1 vial OGYE Selective Supplement. Mix well and pour into plates.

Final pH: 6.6 + 0.2 at 25°C.

The prepared medium is clear and slightly yellowish-brown in color.

Experimental Procedure and Evaluation
 The plates are inoculated using the pour-plate method or the surface spreading method.

Incubation: up to 5 days at 20-25°C.

Count the number of colonies per plate. Calculate the dilution factor into the final count for the sample tested.

Additives

EMD Cat. No. Product Pack Size
1.09877.0001 OGYE Selective Supplement 15 vials
     


Quality control

Test strains
 Growth
Candida albicans ATCC 10231
 		Good to Very Good
Microsporum canis ATCC 36299
 		Fair to Good
Penicillium commune ATCC 10428
 		Good to Very Good
Aspergillus niger ATCC 16404
 		Good to Very Good
Escherichia coli ATCC 25922
 		None
Pseudomonas aeruginosa ATCC 27853
 		None
Bacillus cereus ATCC 11778
 		None


Literature

MOSSEL, D.A.A., VISSER, M., a. MENGERINK, W.H.J.: A comparison of media for the enumeration of molds and yeasts in foods and beverages. - Lab. Pract., 11; 109-112 (1962).
MOSSEL, D.A.A., KLEYNEN-SEMMELING, A.M.C., a. VENCENTE, H.M.: Oxytetracycline-Glucose-Yeast Extract Agar for selective enumeration of molds and yeasts in foods and clinical material. - J. Appl. Bact., 33; 454-457 (1970).


Aspergillus niger, Candida albicans




© 2002 Merck KGaA, Darmstadt, Germany