Buffered Peptone Water

Buffered Peptone Water

EMD Cat. No. 1.07228.0500/5007

500 g, 5 kg

For the preliminary, non-selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae, from foodstuffs and other materials.

This culture media complies with the recommendations of the International Standard Organization ISO (1993) and the German DIN Norms 10181 and 10160 for the examination of milk and meat/meat products respectively.

Mode of Action	Preparation
Experimental Procedure and Evaluation	Quality Control
Typical Composition (g/liter)	Literature

Mode of Action
The broth is rich in nutrients and produces high resuscitation rates for subletally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage due to changes in the pH of the medium.

Typical Composition (g/liter)
Peptone 10.0; sodium chloride 5.0; disodium hydrogen phosphate dodecahydrate 9.0; potassium dihydrogen phosphate 1.5.

Preparation
Suspend 25.5 g/l, if desired, dispense into suitable containers, autoclave (15 min at 121°C).
pH: 7.2 ± 0.2 at 25°C.
The prepared broth is clear and yellowish.

Experimental Procedure and Evaluation
Inoculate the culture medium with the sample material.
Incubation: 16 - 20 hours at 37°C aerobically.

Transfer material from the resulting culture to a selective enrichment culture medium recommended by the appropriate standard.

Quality control

Test strains	Growth
Salmonella typhimurium ATCC 14028	good / very good
Escherichia coli ATCC 25922	good / very good
Staphylococcus aureus ATCC 25923	good / very good
Enterococcus faecalis ATCC 33186	good / very good
Pseudomonas aeruginosa ATCC 27853	good / very good
Streptococcus pyogenes ATCC 12344	good / very good
Bacillus subtilis ATCC 6633	fair / good

Literature

DIN Deutsches Institut für Normung e.V.: Mikrobiologische Milchuntersuchung. Nachweis von Salmonellen. Referenzverfahren. DIN 10181.
DIN Deutsches Institut für Normung e.V.: Untersuchung von Fleisch und Fleischerzeugnissen. Nachweis von Salmonellen. Referenzverfahren. DIN 10160.
International Standard Organisation: Detection of salmonellae (Reference method). International Standard ISO 6579 (1993).
International Standard Organisation: Milk and Milk Products Detection of Salmonella spp. ISO 6785 / IDF 93 (2001)