Brilliant Green Phenol Red Lactose Agar (USP) (Brilliant-green Phenol-red Lactose Sucrose Agar)

Brilliant Green Agar

EMD Cat. No. 1.07232.0500

500 g

Selective agar for the isolation of Salmonella with the exception of S. typhosa and Shigella from pathological materials, faeces, urine, foodstuffs, pharmaceutical materials, etc.

This medium complies with the recommendations of the United States Pharmacopeia XXIII (1995) and the European Pharmacopeia II.

Mode of Action	Preparation
Experimental Procedure and Evaluation	Quality Control
Typical Composition (g/liter)	Literature

Mode of Action
See 7237 Brilliant Green Phenol Red Lactose Agar.

Typical Composition (g/liter)
Peptone from meat, peptic 5.0; peptone from casein 5.0; yeast extract 3.0; sodium chloride 5.0; lactose 10.0; sucrose 10.0; phenol red 0.08; brilliant green 0.0125; agar-agar 13.0.

Preparation
Suspend 51 g/liter, autoclave (15 min at 121 °C), pour plates.
pH: 6.9 ± 0.2 at 25 °C.
The plates are clear and red-brown.

Experimental Procedure and Evaluation
See 7237 Brilliant Green Phenol Red Lactose Agar.

Quality control

Test strains	Recovery rate (%)	Colony color	Medium color
Salmonella typhimurium ATCC 14028	40	red	red
Salmonella choleraesius ATCC 13312	40	red	red
Salmonella enteritidis NCTC 5188	40	red	red
Escherichia coli ATCC 25922	not limited	yellow	yellow
Staphylococcus aureus ATCC 25923	0.01
Enterococcus faecalis ATCC 33186	0.01
Bacillus subtilis ATCC 6633	0.01

Literature

European Pharmacopeia II, Chapter VIII. 10.
United States Pharmacopeia XXIII, Chapter "Microbial Limit Tests", 1995.