SABOURAUD-2 % Dextrose Agar

SABOURAUD 2%-Dextrose Agar

EMD Cat. No. 1.07315.0500/5000

500 g, 5 kg

The medium was recommended by JANKE (1961) for the cultivation of dermatophytes. GEORG et at. (1954) recommended the addition of cycloheximide, penicillin and streptomycin for the inhibition of non-pathogenic accompanying bacteria.

Additives

EMD Cat. No.	Product	Pack Size
1.06993.0010	Penicillin G potassium salt
1.10117.0025	Streptomycin sulfate

Preparation	Experimental Procedure and Evaluation
Quality Control	Typical Composition (g/liter)
Literature

Preparation
Suspend 47 g/liter, autoclave (15 min at 121 °C).

Do not overheat.
pH: 5.6 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.

Experimental Procedure and Evaluation
The plates are inoculated with sample material according to the instructions. The fungi colonies which have grown are judged macro- and microscopically.
Incubation for up to 7 days at 28 °C aerobically.

For preparation of version acc. to EMMONS adjust pH to
6.9 ± 0.2. Chloramphenicol (50 mg/l) may be added in addtion.

Quality control

Test strains	Growth
Trichophyton mentagrophytes	good / very good
Trichophyton rubrum	fair / good
Microsporum gallinae ATCC 121088	good / very good
Trichophyton ajelloi	fair / very good
Microsporum canis	good / very good
Geotrichum candidum DSM 1240	good / very good
Candida albicans ATCC 10231	good / very good
Aspergillus niger	good / very good
Penicillium commune ATCC 10428	good / very good

Typical Composition (g/liter)
Peptone 10.0; D(+)glucose or maltose 20.0; agar-agar 17.0.

Literature

GEORG, L.K., AJELLO, L., a. PAPAGEORGE, C.: Use of cycloheximide in the selective isolation of fungi pathogenic to man. - J. Lab. Clin. Med., 44; 422-428 (1954).
JANKE, D.: Pilznährboden nach SABOURAUD, modifiziert MERCK, ein neuer Trockennährboden zur Züchtung von Dermatophyten. - Zschr. Haut- u. Geschl.-Krankh., 15; 188-193 (1961).