Staphylococcus Broth Base BAIRD

EMD Cat. No. 1.07899.0500
500 g


For the selective enrichment of coagulase-positive staphylococci acc. to § 35 LMBG (German food inspection legislation).



Additives


EMD Cat. No. Product Pack Size
1.05164.0100 Potassium tellurite trihydrate 100 g



Mode of Action Preparation
Experimental Procedure and Evaluation Quality Control
Typical Composition (g/liter) Literature
Picture/Image


Mode of Action
 Lithium chloride and potassium tellurite inhibit most of the accompanying flora, whereas the remaining components of the culture medium permit satisfying growth of staphylococci.

Preparation
Suspend 43 g/liter, fill into test tubes, autoclave (15 min at
121 °C). Prior to use add 0.1 ml filter-sterilized 1 % potassium tellurite solution to 9 ml of base medium below 45 °C.  
pH: 6.6 ± 0.2 at 25 °C.
The prepared culture medium base can be stored in a refrigerator for up to one month. It is clear and yellowish-brown.

Experimental Procedure and Evaluation
According to § 35 LMBG inoculate 1 ml of sample material in
9 ml of culture medium. Incubate up to 48 hours at 35 °C.
Quality control

Test strains
 Inoculum
 		Growth after 24 hours
Escherichia coli ATCC 25922
 		approx. 99 %
 		5 %
Staphylococcus aureus ATCC 6538-P
 		approx. 1 %
 		95 %

Typical Composition (g/liter)
 Peptone from meat 8.0; peptone from casein 2.0; yeast extract 1.0; meat extract 5.0; sodium pyruvate 10.0; glycine 12.0; lithium chloride 5.0.
Literature

Amtliche Sammlung von Untersuchungsverfahren nach § 35 LMBG; Untersuchung von Lebensmitteln. Bestimmung koagulase-positiver Staphylokokken in Trockenmilcherzeugnissen und Schmelzkäse. L 02.07.


Pseudomonas aeruginosa, Staphylococcus epidermis, Staphylococcus aureus



© 2002 Merck KGaA, Darmstadt, Germany