SABOURAUD-2 % Dextrose Broth

SABOURAUD 2%-Dextrose Broth

EMD Cat. No. 1.08339.0500/5000

500 g, 5 kg

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Preparation	Experimental Procedure and Evaluation
Quality Control	Typical Composition (g/liter)
Reference

Typical Composition (g/liter)
Peptone from meat 5.0; peptone from casein 5.0; D(+)glucose 20.0

Preparation
Suspend 30 g/liter, if necessary dispense into smaller vessels, autoclave (15 min at 121 °C).
pH: 5.6 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.

Experimental Procedure and Evaluation
Depend on the purpose for which the culture medium is used.
Incubation: up to 7 days at 28 °C aerobically.

TD STYLE="width:30%" ID="S15"> good / very good

Test strains	Inoculum	Growth
Trichophyton mentagrophytes ATCC 18748		good / very good
Trichophyton rubrum ATCC 28188		fair / good
Microsporum gallinae ATCC 12108		fair / good
Trichophyton ajelloi ATCC 28454		fair / good
Candida albicans ATCC 10231	≤ 100	fair / very good
Aspergillus niger ATCC 16404		good / very good
Penicillium commune ATCC 10428

Reference

United States Pharmacopeia 30

<61> Microbiological Examination of Non-sterile Products: Microbial Enumeration Tests (USP 30, page 89).
<62> Microbiological Examination of Non-sterile Products: Tests for Specified Microorganisms (USP 30, page 93).

European Pharmacopeia 6 (Ph. Eur)
2.6.12 Microbiological Harmonization of Non-sterile Products - Microbial Enumeration Test
2.6.12 Microbiological Harmonization of Non-sterile Products - Tests for Specified Microorganisms