Salmosyst Broth Base

Salmosyst^® Broth Base

EMD Cat. No. 1.10153.0500

500 g

For the two-step enrichment of sublethally injured salmonellae, especially from foodstuffs and feeds.

Mode of Action	Preparation
Quality Control	Typical Composition (g/liter)
Literature

Mode of Action
All the microorganisms present in the sample material are anriched during non-selective pre-enrichment in Salmosyst^® Broth Base. Following the addition of the selctive reagents in the form of a Salmosyst^® Selective Supplement tablet, the growth of the accompanying organisms is inhibited, but the salmonellae continue to grow.

Preparation of the Broth Base
Dissolve 25 g/liter, autoclave (15 min at 121 °C). A visible white precipitation of calcium carbonate which then appears does not affect the performance of the broth. During long storage of the prepared broth some of the calcium carbonate dissolves and can lead to a minimal increase in pH.

Preliminary Enrichment
Suspend 25 g sample material (if necessary homogenize) in 225 ml broth base and incubate for 6-8 hours at 35 °C. Transfer 10 ml of the culture to a sterile test tube.

Selective Enrichment
Add one tablet of Salmosyst^® Selective Supplement to the 10 ml of the preliminary enrichment culture and leave to stand for 30 minutes. Shake vigorously and then incubate for further 18-22 hours at 35 °C. In order to detect the salmonellae, streak a sample of the resulting enrichtment culture onto appropriate selective culture media. Identify the resulting colonies.

Quality control (incl. supplement)

Test strains	Growth
Salmonella typhimurium ATCC 14028	good
Salmonella dublin ATCC 15480	good
Escherichia coli ATCC 25922	inhibited
Enterococcus faecalis ATCC 19433	inhibited

Composition of the Broth Base (g/liter)
Peptone from casein 5.0; peptone from meat 5.0; sodium chloride 5.0; calcium carbonate 10.0.
pH: 7.1 ± 0.2 at 25 °C.

Composition of the Selective Supplement (g/liter)
Potassium tetrathionate 0.2; ox bile 0.08; brilliant green 0.0007; calcium carbonate 0.1.

Literature

WEBER, A.: Über die Brauchbarkeit von Salmosyst^® zur Anreicherung von Salmonellen aus Kotproben von Tieren. - Berl. Münch. Tierärztl. Wschr., 101; 57-59 (1988).
OSSMER, R.: Salmosyst^® and RAMBACH-Agar. A Rapid Alternative for the Detection of Salmonella. Congress-Poster - Salmonella and Solmonellosis - Ploufragan/Saint-Brieux - France, Sepetember 1992.