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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
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A colorimetric sandwich ELISA method for detecting Cu/Zn superoxide dismutase (SOD) in different types of biological samples.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Applications |
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| Do not freeze | Yes | ||
| Sensitivity | 40 pg/ml | ||
| Assay time | 1.5 h | ||
| Sample type | Cell culture supernatant, serum, plasma, urine, amniotic fluid, and other biological fluids | ||
| Kit contains | Pre-Coated 96-Well Plate, Anti-Cu/Zn SOD/HRP-Conjugate, Cu/Zn SOD Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Phosphate Buffered Saline, Substrate Solution, Blue and Green Dyes, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 36/37/38 Irritating to eyes, respiratory system and skin. |
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| Data | |||
|---|---|---|---|
 Recombinant Cu/Zn SOD was diluted in serial two-fold steps in PBS. The mean of three parallel titrations are plotted.    Data represents the mean Cu/Zn SOD concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation was determined to be 5.1%.  Data represents the mean Cu/Zn SOD concentration and the coefficient of variation calculated on 18 determinations of each sample. The overall inter-assay coefficient of variation was determined to be 5.8%.  Percent recoveries of expected values. |
