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Recognizes the ~47 kDa GSK-3β protein phosphorylated at Ser9.
| Accessories forPhosphoDetect™ Anti-GSK-3β (pSer9) (Ab-1) Rabbit pAb Rabbit pAb |
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Ser9 phosphorylation site of human GSK-3β
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Form
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Liquid
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Formulation
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In Dulbecco's PBS (without Mg2+ or Ca2+), 1 mg/ml BSA, 50% glycerol.
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Species reactivity
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human, rat, Xenopus
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Preservative
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≤0.1% sodium azide
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Applications
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Enzyme-Linked Immunosorbent Assay
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Dot Blot
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Immunoblotting (Western Blotting)
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Avoid freeze/thaw
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Yes
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Store and ship information
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Storage
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-20°C
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Ship
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Dry Ice Only
Standard Handling
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Data
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Detection of human GSK-3β by immunoblotting. Sample: Whole cell lysate from control cells (lane 1) or control cell lysate spiked with recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3β (pSer<sup>9</sup>) (Ab-1) Rabbit pAb (Cat. No. PC242) (1:1000) pre-incubated without peptide (lane 1, 5), or with phosphopeptide immunogen (lane 2), non-phosphopeptide corresponding to the immunogen (lane 3), or a generic phosphoserine-containing peptide (lane 4). Detection: chemiluminescence.
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