PS1018
PhosphoDetect™ Anti-GSK-3β, (pSer9) Rabbit pAb
PhosphoDetect™ Anti-TPK1 (pSer9), PhosphoDetect™ Anti-Tau Protein Kinase 1 (pSer9), PhosphoDetect™ Anti-Glycogen Synthase Kinase-3β (pSer9)
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
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Recognizes the ~47 kDa GSK-3β protein phosphorylated on Ser9 in insulin or IGF-1 treated, serum-starved 3T3-L1 cells.
| Substitutes | |
|---|---|
| 361527 | PhosphoDetect™ Anti-GSK3β (pSer9) Mouse mAb (2D3) |
| Substitutes for PhosphoDetect™ Anti-GSK-3β, (pSer9) Rabbit pAb | |
| 361527 | PhosphoDetect™ Anti-GSK3β (pSer9) Mouse mAb (2D3) |
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser9 phosphorylation site of human GSK-3β | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3 (±0.1). | ||
| Species reactivity | human, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Serum-starved 3T3-L1 cells treated with 100 nM insulin or 100 ng/ml IGF-1 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 Detection of human GSK-3β, phosphorylated at Ser<sup>9</sup>, by immunoblotting. Samples: Control lysate (lane 1) or control lysated spiked with recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3β (pSer<sup>9</sup>) Rabbit pAb (Cat. No. PS1018) (1:1000) preincubated without peptide (lanes 1,5) or with phosphopeptide immunogen (lane 2), non-phosphopeptide corresponding to the immunogen (lane 3), or a generic phosphoserine-containing peptide (lane 4). Detection: chemiluminescence. |
