PK1008
PhosphoDetect™ Anti-PDGFRβ(pTyr75¹) Rabbit pAb
Anti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr75¹)
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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
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Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG1 | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr75¹ phosphorylation site of human PDGF receptor-β, coupled to KLH | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | None | ||
| Applications |
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| Positive control | Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of mouse and human PDGFR-β, phosphorylated on Tyr<sup>751</sup>, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr<sup>751</sup>) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.  Detection of human PDGFR-β, phosphorylated on Tyr<sup>751</sup>, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr<sup>751</sup>) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.  Detection of mouse PDGFR-β, phosphorylated on Tyr<sup>751</sup>, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr<sup>751</sup>) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence. |
