A naturally-occurring antagonist to angiopoietin-1. Closely related to the pro-angiogenic factor Ang-1 and binds with similar affinity to the Tie-2/Fc receptor tyrosine kinase. Competitively inhibits Ang-1-induced endothelial cell responses mediated by Tie-2/Fc. Disrupts vasculogenesis in developing embryos by antagonizing Ang-1.
| Product information | |||
|---|---|---|---|
| Form | Solid | ||
| Formulation | Lyophilized from sterile-filtered PBS, 50 µg BSA/µg Ang-2. | ||
| Molar mass | 66000 | ||
| Activity | 0.5-100 ng/ml is the linear range of binding of angiopoietin-2 to Tie-2/Fc. Assay was performed with immobilized Tie-2/Fc (4 µg/ml). | ||
| Biological activity | Linear range of biotinylated angiopoietin-2 binding = 0.5-100 ng/ml based on its ability to bind immobilized Tie-2/Fc (plated at ~400 ng/well) in a functional ELISA. | ||
| Source | cDNA sequence corresponding to human angiopoietin-2 was expressed with a C-terminal 6X histidine tag in a mouse myeloma cell line, NSO, and then biotinylated. | ||
| Solubility | PBS | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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