For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
07 February 2012
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This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product.
Recombinant, human, wild-type BRAC1 (amino acids 1-1863) fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda, insect cells using a baculovirus expression system. Purified by affinity chromatography using FPLC. BRCA1 is a tumor suppressor protein involved in the homologous recombination pathway for DNA double-strand break (DSB) repair. Although evidence suggests that BRCA1 and BRCA2 appear to participate in different protein complexes and may play quite distinct roles in DSB repair, both BRCA proteins have been shown to interact with p53 (Cat. No. 506147) and RAD51 (Cat. No. 553504). Individuals carrying BRCA mutations are predisposed to breast cancer.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | BRCA1 in 100 mM KCl, 20 mM Tris-HCl, 10 mM 2-mercaptoethanol, 0.2 mM EDTA, 20% glycerol, pH 7.9 and Dilution Buffer A, 100 mM KCl, 20 mM Tris-Cl, 20% Glycerol, 1 mM DTT and 0.2 mM EDTA, pH 8.0. | ||
| Molar mass | 205000 | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit equals 1 ng of purified protein. One unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assays; 100 units are sufficient for protein-protein interaction assays. | ||
| Purity | ≥95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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