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324831  EGF, Human, Recombinant, E. coli

Epidermal Growth Factor, Human, Recombinant, E. coli

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04 February 2012

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324831-200UG  200 μg 
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Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
Product information
Form Solid
Formulation Lyophilized from sterile-filtered PBS.
Molar mass 6000
Biological activity ED50 = 100-400 pg/ml as measured by its ability to stimulate the 3H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Purity ≥95% by SDS-PAGE
Contaminants Endotoxin: <1.0 EU/μg EGF
Solubility 10 mM acetic acid containing ≥0.1% HSA or BSA
RTECS KB4518000
CAS number 62229-50-9
Merck USA index 14, 3527
Store and ship information
Storage -20°C
Ship Ambient Temperature Only
Standard Handling

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© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.

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