324831
EGF, Human, Recombinant, E. coli
Epidermal Growth Factor, Human, Recombinant, E. coli
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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: emdinfo@emdchemicals.com
03 September 2010
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Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
| Product information | |||
|---|---|---|---|
| Form | Solid | ||
| Formulation | Lyophilized from sterile-filtered PBS. | ||
| Molar mass | 6000 | ||
| Biological activity | ED50 = 100-400 pg/ml as measured by its ability to stimulate the 3H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3. | ||
| Purity | ≥95% by SDS-PAGE | ||
| Contaminants | Endotoxin: <1.0 EU/μg EGF | ||
| Solubility | 10 mM acetic acid containing ≥0.1% HSA or BSA | ||
| RTECS | KB4518000 | ||
| CAS number | 62229-50-9 | ||
| Merck USA index | 14, 3527 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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