Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
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Product information
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Form
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Solid
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Formulation
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Lyophilized from sterile-filtered PBS.
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Molar mass
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6000
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Biological activity
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ED50 = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
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Purity
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≥95% by SDS-PAGE
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Contaminants
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Endotoxin: <1.0 EU/μg EGF
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Solubility
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10 mM acetic acid containing ≥0.1% HSA or BSA
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RTECS
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KB4518000
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CAS number
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62229-50-9
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Merck USA index
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14, 3527
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Store and ship information
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Storage
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-20°C
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Ship
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Ambient Temperature Only
Standard Handling
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