Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
| Accessories for PhosphoDetect™ Anti-EGFR (pTyr845) Rabbit pAb |
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr845 phosphorylation site of human EGFR
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Form
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Liquid
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Formulation
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In Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
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Species reactivity
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human
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Preservative
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≤0.1% sodium azide
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Applications
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-
Immunoblotting (Western Blotting)
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Avoid freeze/thaw
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Yes
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Positive control
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A431 cells stimulated with EGF
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Standard Handling
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Data
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Detection of human epidermal growth factor receptor, phosphorylated on Tyr<sup>845</sup>, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr<sup>845</sup>) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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