324867
PhosphoDetect™ Anti-EGFR (pTyr¹068) Rabbit pAb
Anti-Epidermal Growth Factor Receptor (pTyr¹068)
Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹068 phosphorylation site of human EGFR | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4. | ||
| Species reactivity | chicken, human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
||
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 Detection of human EGFR, phosphorylated at Tyr<sup>1068</sup>, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr<sup>1068</sup>) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence. |
