324869
PhosphoDetect™ Anti-EGFR (pTyr¹¹48) Rabbit pAb
Anti-Epidermal Growth Factor Receptor (pTyr¹¹48)
Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1148. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹¹48 phosphorylation site of human EGFR | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS, 50% glycerol, 1 mg/ml BSA, pH 7.2-7.4. | ||
| Species reactivity | chicken, human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 Detection of human EGFR, phosphrylated at Tyr<sup>1148</sup>, by immunoblotting. Sample: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lane 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr<sup>1148</sup>) Rabbit pAb (Cat. No. 324869) (1:1000) pre-incubated without peptide (lanes 1, 2), with non phosphopetide corresponding to the immunogen (lane 3), a generic phosphotyrosine containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Detection: chemiluminescence. |
