Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
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Product information
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Form
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Lyophilized
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Formulation
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Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
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Molar mass
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16400
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Biological activity
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ED50 = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
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Purity
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≥97% by SDS-PAGE
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Contaminants
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Endotoxin: ≤1 EU/µg FGF
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Solubility
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Sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA
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RTECS
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LK3641325
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CAS number
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106096-93-9
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Merck USA index
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14, 4072
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Declaration
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Sold under license of U.S. Patent 5,155,214.
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Ambient Temperature Only
Standard Handling
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Canadian export regulations
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Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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