341595
FGF-2, Basic, Human, Recombinant, E. coli
rhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: emdinfo@emdchemicals.com
03 September 2010
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Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Formulation | Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0. | ||
| Molar mass | 16400 | ||
| Biological activity | ED50 = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent 3H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts | ||
| Purity | ≥97% by SDS-PAGE | ||
| Contaminants | Endotoxin: ≤1 EU/µg FGF | ||
| Solubility | Sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA | ||
| RTECS | LK3641325 | ||
| CAS number | 106096-93-9 | ||
| Merck USA index | 14, 4072 | ||
| Declaration | Sold under license of U.S. Patent 5,155,214. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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