361526
GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli
GSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
Note: 1 KU = 1000 units.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5 | ||
| Molar mass | 47000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥5,000,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl. | ||
| Activity | ≥250,000 units/ml | ||
| Contaminants | DNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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