For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
07 February 2012
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Recognizes the ~47 kDa GSK-3β protein in a variety of rat and mouse tissues as well as in human thymus and HeLa cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide (CAHSFFDELRDPNVK) corresponding to amino acids 334-348 of rat glycogen synthase kinase 3β kinase subdomain XI, conjugated to KLH | ||
| Form | Liquid | ||
| Formulation | In PBS, 50% glycerol, pH 7.2. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Rat or mouse tissues, human thymus or HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human GSK-3β by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), Jurkat cells (lane 3), 3T3 cells (lane 4), PC-12 cells (lane 5), and MDBK cells (lane 6). Primary antibody: Anti-GSK-3β, C-Terminal (334-348) Rabbit pAb (Cat. No. 361528) (1 µg/ml). Detection: chemiluminescence.  Detection of human GSK-3β by immunohistochemstry. Sample: Human hepatoma tissue. Primary antibody: Anti-GSK-3β, C-Terminal (334-348) Rabbit pAb (Cat. No. 361528) (assay dependent). Detection: fluorescence. |
