The ProteoExtract® Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue. The isolated cells can then be used for total, subcellular, membrane, or other form of proteome extractions. This kit serves as an accessory product for the following kits: ProteoExtract® Subcellular Proteome Extraction Kit (Cat. No.: 539790), ProteoExtract® Subcellular Proteome Extraction Kit, Mini (Cat. No.: 539791), ProteoExtract® Native Membrane Protein Extraction Kit (Cat. No.: 444810).
| Product information | |||
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| Form | 10 Dissociation reactions, 1 g each | ||
| Sample type | Fresh tissue samples from eukaryotic organisms | ||
| Kit contains | 10X Tissue Dissociation Buffer, Protease Inhibitor Cocktail, Tissue Dissociation Buffer Additive and a user protocol. | ||
| Store and ship information | |||
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<b>Hazardous Materials Attention:</b> Due to the nature of the Hazardous Materials in this shipment, additional shipping charges <i>may</i> be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
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  Tissue dissociation of rat pancreatic tissue and human pancreatic tissue was carried out according to the protocol outlined above. 1.3 x 10<sup>7</sup> dissociated cells were extracted using the ProteoExtract<sup>®</sup> Subcellular Proteome Extraction Kit protocol (Cat. No. 539790), incorporating the protocol changes as outlined above. Proteins from the cytosolic fraction, the membrane fraction, the nuclear fraction, and the cytoskeletal fraction were separated by SDS-PAGE, transferred to PVDF membrane, and probed with antibodies to the indicated markers. |
