69049
Pellet Paint® Co-Precipitant
Rapid, quantitative precipitation of DNA and RNA, including PCR clean-up
Pellet Paint® Co-Precipitant is a visible dye-labeled
carrier formulated specifically for
use in alcohol precipitation of nucleic acids
(McCormick 1995, McCormick 1996). The two-minute
precipitation uses just 2 µl per reaction
and requires no low-temperature incubations
or prolonged centrifugation. Both RNA and
DNA are efficiently precipitated from even
dilute solutions (2 ng/ml) and the pellet is
easily located by its vivid pink color. The
pellet can be easily followed during washing
steps and prevents losses during handling.
Most applications of PCR benefit from
a clean-up step in which primers and other
reactants are removed and the target DNA is
concentrated (Taggart 1998). Pellet Paint Co-
Precipitant is ideal for this cleanup because
the procedure is rapid, primers < 50 nt in
length are efficiently removed, and the DNA
is quantitatively recovered. Furthermore,
it is easy to tell when the DNA has been
completely resuspended following the
precipitation step.
Pellet Paint Co-Precipitant is compatible
with most molecular biology procedures and
is free of contaminating nucleic acids and
nucleolytic enzymes. Although it absorbs in
the UV range, accurate spectrophotometric
measurements of DNA or RNA samples are
possible; the absorbance ratio (provided with
each package) can be used as a correction
factor when determining nucleic acid
concentration (McCormick 1996). Pellet Paint
Co-Precipitant is compatible with automatted
Cy5® sequencers. Pellet Paint NF Co-
Precipitant is recommended for use with PE
Applied Biosystems automated sequencers.
Note: Euorpean Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending.
| Product information | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Form | Liquid | ||||||||
| Components |
|
||||||||
| Features and benefits |
• Direct visualization and tracking of
precipitated material |
||||||||
| Declaration | patent pending | ||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
||
