Because the translation efficiency and fidelity of initiation of particular RNAs depend on the salt concentrations and counter ions used in the reaction, magnesium and potassium solutions are provided separately. The system includes KCl as the salt of choice for pCITE® transcripts and higher fidelity translation of capped mRNAs and KOAc for improved performance of uncapped messages (Jackson 1991). A unique Control RNA is included, which permits monitoring of translation performance and yields a set of 6 protein size markers when translated in vitro. One domain of the initial translation product is a specific protease that cleaves the rest of the molecule into discrete peptides during the translation reaction. Their final sizes are 6.3, 21.8, 30.3, 52.0, 73.7, and 83.3 kDa, which are convenient sizes for accurate gel analysis of experimental samples.
| Product information | |||||||||||||||||||||||||||||||||
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| Avoid freeze/thaw | Yes | ||||||||||||||||||||||||||||||||
| Components |
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| Features and benefits |
• Highest activity, lowest background available • All reaction components except labeled amino acid and RNA sample are provided • Separate magnesium and choice of potassium salts enable maximal performance with capped, pCITE, and uncapped RNAs • Concentrated lysate comprises only 40% of reaction volume |
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| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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