The SpinPrep™ PCR Clean-Up Kit is designed
for the rapid purification of DNA amplified
in PCR. The 10-minute procedure involves
addition of a binding buffer followed by
adsorption of the DNA to a silica membrane in
a spin column format. Following a wash step,
the DNA is eluted in low-salt buffer. This kit
removes DNA polymerases, dNTPs, salts, and
> 99% of primers so that they do not interfere
with downstream applications such as cloning,
sequencing, or labeling. PCR products from
100 bp to > 12,000 bp can be cleaned up, with
recoveries of amplified DNA in the range of
60-90% under standard conditions.
| |
|
| Column binding capacity: |
up to 6 µg |
| PCR volume: |
100 µl/rxn |
| Typical recovery: |
60–90% |
| Size range: |
100 bp to > 12,000 bp |
| Time required: |
< 10 min |
|
Product information
|
|
Components
|
| • | 82 ml | SpinPrep Bind Buffer
| | • | 27 ml | SpinPrep Wash Buffer
| | • | 10 ml | SpinPrep Elute Buffer
| | • | 100 | SpinPrep Filters
| | • | 100 | Receiver Tubes
| | • | 100 | Eluate Receiver Tubes |
|
|
Store and ship information
|
|
|
|
Storage
|
+15°C to +30°C
|
|
Ship
|
Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS
|
