Overnight Express™ Instant TB Medium is a granulated autoinduction culture medium for high-level protein production in pET and other IPTG-inducible bacterial expression systems. Instant TB Medium is a combination of Overnight Express Autoinduction System 1 components (Cat. No. 71300) with rich Terrific Broth (TB) medium and is supplied in two formats.
EasyPak is an aluminum foil pouch containing 60 g granulated medium, sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min to dissolve. Overnight Express Instant TB Medium is also available in 1 kg bottles. Granulated medium ensures rapid, uniform dissolution in water, prevents clumping of the medium, and avoids inhalation of airborne powder.

Overnight Express™ Autoinduction media are based on technology developed by F. William Studier at Brookhaven National Laboratory and enable regulated protein expression in E. coli,without monitoring the culture or adding IPTG during cell growth. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique autoinduction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems.

Cell mass and target protein yield are often increased several-fold compared to conventional protocols using induction with IPTG. Autoinduction media are extremely convenient for routine expression of proteins in multiple cultures and are ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min. Overnight Express Instant TB Medium is also available in 1 kg bottles.

In addition to Instant TB Medium, a variety of Overnight Express Autoinduction Media are available for different methods:
Overnight Express™ Autoinduction System 1 (71300) contains 3 sterile solutions, which can be added to your choice of glucose-free bacterial growth medium.

Overnight Express™ Autoinduction System 2 (71366) prepares a defined bacterial growth medium that can be used to selenomethionine-label proteins for x-ray crystallography.

Overnight Express Autoinduction NMR Media are 3 different media designed for cell growth Optimization (71760), 15N (71759) protein labeling, and 15N, 13C (71789) dual protein labeling for NMR analysis.