The Novagen® WideScreen™ Group has developed immunoassay panels using a novel, powerful assay platform for the multiplex quantitation of phosphorylated proteins. WideScreen EpiTag™ Assays for the bead-based xMAP® platform utilize EpiTag™ Technology, licensed from Epitome Biosystems Inc., which detects protein- and phosphoprotein fragments rather than the traditional detection of intact proteins. Protein fragments are released by digestion with site-specific proteases and antibody pairs are generated to unique amino acid sequences, or EpiTag™ Sequences, on these fragments. This approach provides a high degree of specificity and the ability to multiplex assays for a protein’s total and phosphorylated levels without cross-reactivity. In addition, the assay can accurately measure concentrations of phosphorylated target proteins as synthetic phosphopeptides are used to generate standard curves.
The first WideScreen EpiTag assays have been developed to detect key signaling events in the ERK pathway from both human and mouse samples. ERK1 and ERK2 are serine/threonine kinases expressed broadly in tissues and various cell lines. ERKs are activated through their phosphorylation by the MAPK/ERK kinases MEK1 and MEK2. Once activated, ERK1 and ERK2 can phosphorylate many different proteins including cytoskeletal proteins, translation regulators, and transcription factors. ERK pathway protein levels and phosphorylation are deregulated in many types of cancer and are considered strong candidates for the development of therapeutic compounds.
The WideScreen EpiTag™ ERK Pathway Panel I Complete Kit contain all lysis, wash and digest buffers, premixed beads, and detection cocktails needed to run 96 tests. The components of the Complete Kits are also available separately, enabling greater options for cell culture and processing and the creation of custom multiplexes (please consult the EpiTag Assay Compatibility Chart) .
WideScreen EpiTag ERK Pathway Panel I Complete Kit Components
Premixed bead kits and detection antibodies for the multiplex quantitation of:
B-Raf ( pS446 )
MEK1/2 ( pS217, pS221/ pS222, pS226 )
ERK1 Total
ERK1 ( pT202/pY204 )
ERK2 Total ERK2 ( pT185/pY187 )
EpiTag™ Cell Lysis Kit
Lysis Reagent, Phosphatase Inhibitor Cocktail Set V, Benzonase® Nuclease HC, Purity > 99%, 100X TCEP Solution, Aluminum Plate Sealer
EpiTag™ Digest Kit
a-Iodoacetamide, Endopeptidase Lys-C
WideScreen™ Reagent Kit
Streptavidin-PE Concentrate, 96-Well Filter Plate and Sealer, 5X Assay Diluent,10X Wash Buffer
Due to the unique nature of the EpiTag Technology and sample preparation, the EpiTag buffers and Bead Kits are incompatible with other Novagen® brand bead-based assay buffers and kits and those from other companies. EpiTag Technology and Assays are offered under limited, non-transferable license from Epitome Biosystems, Inc.
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Blue or Dry Ice
Toxic |
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S: 22-23-24/25-26-36/37/39-45-A09 Do not breathe dust. Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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R: 20/21/22-25-36/37/38-43 Toxic if swallowed. May cause sensitization by skin contact. |
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