The EpiTag™ Cell Lysis Kit contains a denaturing and reducing cell Lysis Reagent that efficiently releases proteins from the cell cytosol, membranes, and nucleus and is for use with the WideScreen™ EpiTag assays. EpiTag™ Technology is licensed from Epitome Biosystems Inc. Benzonase ® Nuclease reduces sample viscosity caused by chromosomal DNA, and a cocktail of phosphatase inhibitors maintains the phosphorylation state of target proteins during cell lysis. An aluminum plate sealer is included for use when processing cells grown in 96-well plates. The kit is sufficient to make 5 ml of cell or tissue lysate (or 160 wells of cells grown in 96-well plates), and contains additional Lysis Reagent to prepare Titration Buffer for use in the WideScreen Assay.
Components
- Lysis Reagent
- Phosphatase Inhibitor Cocktail Set V (50X)
- Benzonase® Nuclease HC, Purity > 99%
- 100X TCEP Solution
- Aluminum Plate Sealer
Due to the unique nature of the EpiTag Technology and sample preparation, the EpiTag buffers and Bead Kits are incompatible with other Novagen® brand bead-based assay buffers and kits and those from other companies. EpiTag Technology and Assays are offered under limited, non-transferable license from Epitome Biosystems, Inc.
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| Storage | -20°C | ||
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Blue or Dry Ice
Toxic |
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| End use certificate | To purchase this product, the completion of a "Select Agent Declaration" and/or "End-Use Certificate" is required. Once your sales order is placed, our Regulatory Specialist will be contacting the end-user of this product to complete the required form(s). Please ensure to provide the customer service representative with the end-user's name, telephone number, fax number and e-mail address to prevent delivery delays. (Note: the completed form must be returned within five (5) business days from receipt and will be valid for one year from the date of signing.) | ||
| Safety information | |||
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| S Phrase |
S: 23-24/25-26-36/37/39 Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
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| R Phrase |
R: 20/21/22-25-36/37/38 Toxic if swallowed. |
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