For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
04 February 2012
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Overnight Express™ Autoinduction NMR-15N, -13C, when supplemented with 13C-labeled glycerol (Cat. No. CLM1510EMD), provides reagents to make 1 L of defined autoinducing liquid culture medium used to produce [U-15N, U-13C]-labeled proteins for NMR spectroscopy. The autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density and to automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG). The system includes four concentrated sterile solutions: OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by induction; OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis; OnEx Solution 3 provides magnesium for maximal cell density; OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels. The kit also includes 15N-labeled solid ammonium chloride, 13C-labeled solid glucose, and reagents to make a non-inducing starter medium, for staged growth without expression and for stable storage of clones. Efficient 13C protein labeling requires 13C-glycerol, which must be purchased separately. Addition of the four liquid components and sterile solutions of 15N ammonium chloride, 13C glucose, and 13C glycerol to sterile water results in a chemically- defined medium promoting high cell densities, autoinduction of expression, and efficient labeling of target proteins.
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| Storage | Multiple storage requirements | ||
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Multiple Storage Conditions
Harmful |
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| S Phrase |
S: 36 Wear suitable protective clothing. |
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| R Phrase |
R: 22-36 Harmful if swallowed. Irritating to eyes. |
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